other:Article Title: Novel antibodies reveal presynaptic localization of C9orf72 protein and reduced protein levels in C9orf72 mutation carriers
Article Snippet: Other primary antibodies used in this study included: anti-C9orf72 (22637–1-AP, Proteintech Group; 66,140–1-Ig, Proteintech Group; GTX119776, GeneTex; sc-138,763, Santa Cruz; AP12928b, Abgent), anti-synaptoporin (102,002, Synaptic Systems), anti-synaptophysin (101,002, Synaptic Systems; M0776, Dako), anti-GAPDH (MAB374, Millipore), anti-α-tubulin (T5168, Sigma), anti-RAB3 (107,003, Synaptic Systems), anti-RAB3A (HPA003160, Atlas Antibodies), anti-PSD-95 (ab18258, Abcam), anti-LAMP1 (ab24170, Abcam), anti-LAMP2 (10397–1-AP, Proteintech Group), anti-SMCR8 (1D2, homemade), anti-RAB39B (1H1, homemade), anti-Histone H3 (ab21054, Abcam), anti-Cox IV (ab16056, Abcam), anti-myc tag (2276 and 2272, Cell Signaling), anti-FLAG tag (PA1-984B, Pierce), and anti-HA tag (26,183, Pierce or 3F10, Merck).
Article Title: Increased level of RAB39B leads to neuronal dysfunction and behavioural changes in mice.
Article Snippet: Antibodies used were anti- RAB39B (D- 12162- 1- AP), anti- GluA2 (11994- 1- AP), anti- GluN2A (19953- 1- AP), anti- GluN2B (19954- 1- AP) and anti- SYN1 (20258- 1- AP) from Proteintech; anti- GluN1 (5704 s), anti- PSD95 (3450 s), anti- β- actin (8457 s), anti- p62 (5114 s), anti- LC3B (3868 s), anti- mTOR (2983 S), anti- phosphorylated mTOR (Ser2448) (5536 S), anti- Akt (9272 S), anti- phosphorylated Akt (Ser473) (9271 S), anti- S6 (2217 s) and anti- phosphorylated S6 (Ser240/244) (5364 s) from Cell Signaling Technology; anti- GluA1 (04– 855), anti- GluA3 (MAB5416) and anti- VGluT1 (MAB5502) from Millipore; and anti- synaptophysin (SYP) (S5768) from Sigma- Aldrich.
Article Title: Increased level of RAB39B leads to neuronal dysfunction and behavioural changes in mice
Article Snippet: Antibodies used were anti‐RAB39B (D‐12162‐1‐AP), anti‐GluA2 (11994‐1‐AP), anti‐GluN2A (19953‐1‐AP), anti‐GluN2B (19954‐1‐AP) and anti‐SYN1 (20258‐1‐AP) from Proteintech; anti‐GluN1 (5704 s), anti‐PSD95 (3450 s), anti‐β‐actin (8457 s), anti‐p62 (5114 s), anti‐LC3B (3868 s), anti‐mTOR (2983 S), anti‐phosphorylated mTOR (Ser2448) (5536 S), anti‐Akt (9272 S), anti‐phosphorylated Akt (Ser473) (9271 S), anti‐S6 (2217 s) and anti‐phosphorylated S6 (Ser240/244) (5364 s) from Cell Signaling Technology; anti‐GluA1 (04–855), anti‐GluA3 (MAB5416) and anti‐VGluT1 (MAB5502) from Millipore; and anti‐synaptophysin (SYP) (S5768) from Sigma‐Aldrich.
Western Blot:Article Title: C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy
Article Snippet: .. Antibodies used in this study included anti-Rab39A (13355, Proteintech), anti-Rab39B (12162, Proteintech), anti-Rab2 (15420, Proteintech), anti-Rab7 (55469, Proteintech), anti-Flag M2 (F3165, Sigma), anti-HA (H3663, Sigma), anti-EGFP (ab6556, Abcam), anti-LC3 (for WB, 7543, Sigma; for IF, PM036 or M152-3, MBL), anti-p62 (H00008878-M01, Abnova), anti-VPS39 (16219, Proteintech), ani-VPS41 (13869, Proteintech), anti-VPS11 (ab189920, Abcam), anti-VPS16 (17776, Proteintech), anti-VPS33A (16896, Proteintech), anti-VPS18 (ab178416, Abcam), anti-C9orf72 (22637, Proteintech), anti-STX17 (HPA001204, Sigma), anti-SNAP29 antibody(sc-135564, Santa Cruz), anti-LAMP2 (sc-18822, Santa Cruz or L0668, Sigma), anti-Myc (2276, Cell Signaling), anti-α -Tubulin (E7, DSHB), anti-VAMP8 (ab76021, Abcam), anti-WIPI2 (Abcam, Ab105459). .. Reagents used in this study included LysoTacker (C1046, Beyotime), Torin 1 (F6101, UBP Bio), CQ (C6628, Sigma), Bafilomycin A1 (S1413, Selleck), human EGF (E9644, Sigma), Doxycycline (D9891, Sigma), GST beads (17-0756-05, Cytivia), IgG beads, (17-0969-01, Cytivia), Flag agarose (A2220, Sigma), HA agarose (A2095, Sigma), DiI (D3911, Invitrogen), DiD (D307, Invitrogen), POPE (850757, Avanti), POPC (850457, Avanti), biotin (60338ES10, Yeasen), StrepTactin XT beads (2-5030-025, IBA), Histodenz (D2158, Sigma).
Article Title: C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy.
Article Snippet: .. Antibodies used in this study included anti-Rab39A (13355, Proteintech), anti-Rab39B (12162, Proteintech), anti-Rab2 (15420, Proteintech), anti-Rab7 (55469, Proteintech), anti-Flag M2 (F3165, Sigma), anti-HA (H3663, Sigma), anti-EGFP (ab6556, Abcam), anti-LC3 (for WB, 7543, Sigma; for IF, PM036 or M152-3, MBL), anti-p62 (H00008878M01, Abnova), anti-VPS39 (16219, Proteintech), ani-VPS41 (13869, Proteintech), anti-VPS11 (ab189920, Abcam), anti-VPS16 (17776, Proteintech), anti-VPS33A (16896, Proteintech), anti-VPS18 (ab178416, Abcam), anti-C9orf72 (22637, Proteintech), anti-STX17 (HPA001204, Sigma), anti-SNAP29 antibody(sc-135564, Santa Cruz), anti-LAMP2 (sc18822, Santa Cruz or L0668, Sigma), anti-Myc (2276, Cell Signaling), anti-α -Tubulin (E7, DSHB), anti-VAMP8 (ab76021, Abcam), anti-WIPI2 (Abcam, Ab105459). .. Reagents used in this study included LysoTacker (C1046, Beyotime), Torin 1 (F6101, UBP Bio), CQ (C6628, Sigma), Bafilomycin A1 (S1413, Selleck), human EGF (E9644, Sigma), Doxycycline (D9891, Sigma), GST beads (17-0756-05, Cytivia), IgG beads, (17-0969-01, Cytivia), Flag agarose (A2220, Sigma), HA agarose (A2095, Sigma), DiI (D3911, Invitrogen), DiD (D307, Invitrogen), POPE (850757, Avanti), POPC (850457, Avanti), biotin (60338ES10, Yeasen), StrepTactin XT beads (2-5030-025, IBA), Histodenz (D2158, Sigma).
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